AOL and Time Warner combined in 2001, but in May, Time Warner stated its intention to spin off AOL into a separate company by the year-end.
Company spokeswoman Tricia Primrose said a voluntary redundancy programme would begin on 4 December.
"We'll need to do an involuntary layoff if we do not reach the target numbers through the voluntary option," she said.
"We believe the voluntary programme gives people more choice and decision-making ability instead of waiting for the final cost recommendations and involuntary layoffs."
AOL bought Time Warner at the very top of the dotcom boom for $147bn, but struggled to integrate the two companies.